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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: FeCl2

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52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

76 GOTTSCHALKIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Uric acid 2 11.897
Na2CO3 1.5 14.153
Yeast extract 1 -
K2HPO4 0.91 5.225
KOH 0.67 11.942
Na-thioglycolate 0.5 4.382
MgSO4 x 7 H2O 0.25 1.014
CaCl2 x 2 H2O 0.015 0.102
FeSO4 x 7 H2O 0.006 0.022
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
Gas phase:
  • 100 % N2

95 DESULFUROMONAS ACETOXIDANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.92 340.868
Na2-DL-malate 2.649 14.88
MgCl2 x 6 H2O 1.992 9.798
NaHCO3 1.843 21.934
Na2SO4 1.763 12.412
KH2PO4 0.996 7.319
MgSO4 x 7 H2O 0.996 4.041
Yeast extract 0.498 -
Na2S x 9 H2O 0.299 1.244
NH4Cl 0.299 5.586
CaCl2 x 2 H2O 0.1 0.677
Na2-EDTA x 2 H2O 0.005 0.015
FeCl2 x 4 H2O 0.001 0.008
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Solutions:
  • 951 ml Solution A
  • 3 ml Solution B
  • 40 ml Solution C
  • 10 ml Solution D
  • 1 ml Trace element solution SL-11
Gas phase:
  • 100 % N2

119 METHANOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
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Solutions:
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

119a METHANOBREVIBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
Yeast extract 0.997 -
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 200 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 80 % H2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

119c GUDONGELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 2 29.409
Na-acetate 1 12.19
NaHCO3 0.5 5.952
KH2PO4 0.5 3.674
L-Cysteine HCl x H2O 0.5 2.847
Na2S x 9 H2O 0.5 2.082
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
HCl 0.003 0.069
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.002 0.008
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 100 % N2

120 METHANOSARCINA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaCl 2.197 37.599
NaHCO3 1.953 23.25
Casitone 1.953 -
Yeast extract 1.953 -
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
K2HPO4 0.342 1.962
L-Cysteine HCl x H2O 0.293 1.668
Na2S x 9 H2O 0.293 1.22
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 100 % N2
  • 20 % CO2

120a METHANOSARCINA BARKERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NaHCO3 0.83 9.881
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
K2HPO4 0.342 1.962
L-Cysteine HCl x H2O 0.293 1.668
Na2S x 9 H2O 0.293 1.22
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

120b METHANOMICROCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaHCO3 2.441 29.062
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
L-Cysteine HCl x H2O 0.352 2.002
Na2S x 9 H2O 0.352 1.464
K2HPO4 0.342 1.962
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
2-Mercaptoethanesulfonate 0.137 0.961
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2
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