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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Choline chloride

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63c DESULFOVIBRIO MEDIUM (CHOLINE)

Compound Conc. [g/L] Conc. [mM]
Choline chloride 5 35.812
MgSO4 x 7 H2O 2 8.114
NH4Cl 1 18.695
Na2SO4 1 7.04
Yeast extract 1 -
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Solutions:
  • 980 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
Gas phase:
  • 100 % N2

540 ACETITOMACULUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Glucose 4.926 27.343
NaHCO3 3.941 46.911
Yeast extract 1.478 -
MgCl2 x 6 H2O 1.182 5.815
KCl 0.493 6.608
NH4Cl 0.296 5.526
L-Cysteine HCl x H2O 0.296 1.683
Na2S x 9 H2O 0.296 1.231
KH2PO4 0.197 1.448
Na2SO4 0.099 0.694
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
NaCl 0.01 0.169
MnSO4 x H2O 0.005 0.029
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
NiCl2 x 6 H2O 9.9e-4 0.008
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 9.9e-5 0.002
Pyridoxine hydrochloride 9.9e-5 4.8e-4
Na2MoO4 x 2 H2O 9.9e-5 4.1e-4
CuSO4 x 5 H2O 9.9e-5 3.9e-4
Na2WO4 x 2 H2O 9.9e-5 3.0e-4
Niacinamide 4.9e-5 4.0e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
Choline chloride 4.9e-5 3.5e-4
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

1494 . OMIZ-PAT Medium (modified)

Compound Conc. [g/L] Conc. [mM]
(NH4)6Mo7O24 x 4 H2O 12.4 -
Yeast extract 3 -
Fe SO4 x 7 H2O 2.78 10
D-Arabinose 2 13.322
L-Rhamnose 2 12.183
L-Fucose 2 12.183
D-Mannose 2 11.102
D-Glucose 2 11.101
D-Trehalose 2 5.843
D-Sucrose 2 5.843
NH4Cl 1.6 29.912
Glutathione 1.537 5
Ethanol 1 21.706
NaHCO3 1 11.904
Ascorbic acid 1 5.678
KCl 0.968 12.985
D-Fructose 0.8 4.441
D-Mannitol 0.8 4.392
D-glucuronic acid 0.8 4.121
D-galacturonic acid 0.8 4.121
D-Maltose 0.8 2.337
L-Glutamine 0.68 4.653
L-Histidine 0.62 3.996
NaVO3 0.61 5.003
D 0.56 4.866
Pyruvic acid 0.55 6.246
L-Serine 0.525 4.996
Fumaric acid 0.5 4.308
HCl 0.365 10
L-Cysteine HCl 0.35 2.221
Formic acid 0.3 6.518
L-Glutamic acid 0.3 2.039
ZnSO4 x 7 H2O 0.287 0.998
MgSO4 x 7 H2O 0.25 1.014
DL-carnitine 0.2 1.241
Citric acid 0.2 1.041
N-Acetylglucosamine 0.2 0.904
L-Lysine HCl 0.182 -
L-Arginine 0.175 1.005
L-Ornithine HCl 0.17 -
L-Phenylalanine 0.165 0.999
CaCl2 x H2O 0.15 1.352
L-Asparagine 0.15 1.135
L-Methionine 0.15 1.005
NaH2PO4 x H2O 0.14 1.167
L-Isoleucine 0.131 0.999
L-Leucine 0.131 0.999
NiSO4 x 6 H2O 0.131 0.846
L-Threonine 0.12 1.007
L-Aspartic acid 0.118 0.887
SnCl2 x 2 H2O 0.118 0.523
L-Valine 0.117 0.999
L-Proline 0.115 0.999
L-Tryptophan 0.102 0.499
Nicotinic acid 0.1 0.812
Folinic acid 0.1 0.211
L-Tyrosine 0.09 0.497
NaOH 0.08 2
Glycine 0.075 0.999
Nicotinamide 0.05 0.409
Choline chloride 0.05 0.358
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Solutions:
  • 200 ml Solution H
  • 8 ml Solution I
  • 2 ml Solution J
  • 40 ml Solution K
  • 2 ml Solution L
  • 200 ml Trace elements III
  • 10 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 10 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 2 ml Trace elements I
  • 2 ml Trace elements II
  • 200 ml Supplement 1
  • 2 ml Supplement 2
  • 2 ml Supplement 3
  • 2 ml Supplement 5
  • 200 ml Supplement 4