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943 SEDIMENTIBACTER SAALENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.8 45.234
Yeast extract 3 -
L-Arginine x HCl 2.11 -
Glycine 0.75 9.991
(NH4)HCO3 0.45 -
Na2S x 9 H2O 0.15 0.625
MgSO4 x 6 H2O 0.13 1.08
CaCl2 x 2 H2O 0.12 0.816
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
D-(+)-biotin 2.0e-5 8.2e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Sodium Potassium phosphate buffer - 10
Distilled water - -
Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2