194: DESULFOBULBUS SP. MEDIUM (FRESHWATER)

Modifications: Na-propionate is replaced by 0.40 g/l 3,4,5-trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution (2% w/v) prepared under N2 and neutralized with NaOH. Stock solutions of 3,4,5-trimethoxybenzoate should be prepared freshly and sterilized by filtration. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration.

Growth observed:

Growth conditions: 3-7 days anaerobic 55 °C