Strains for SULFOLOBUS MEDIUM

Name Growth Modifications External links
Archaeon Acidianus sp. DSM 29038 2-3 days | 70 °C Supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. DSMZ BacDive
Archaeon Acidianus sp. DSM 29099 3-7 days | 65 °C Use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 - 3.0. DSMZ BacDive
Archaeon Acidianus sulfidivorans DSM 18786 70 °C Use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150°C overnight. Adjust the pH of the medium to 0.8. DSMZ BacDive
Bacterium Laceyella sediminis DSM 45263 55 °C | 7.50 (pH) | aerobic Adjust pH of medium to 7.5 DSMZ BacDive
Archaeon Metallosphaera hakonensis DSM 7519 3-7 days | 65 °C Adjust pH of medium to 3.0 - 3.5. DSMZ BacDive
Archaeon Metallosphaera javensis DSM 112778 (+) 2-3 days | 70 °C Supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. Adjust the pH of the medium to 3.0. DSMZ BacDive
Archaeon Metallosphaera prunae DSM 10039 75 °C Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. DSMZ BacDive
Archaeon Metallosphaera sedula DSM 5348 3-7 days | 65 °C Omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. DSMZ BacDive
Archaeon Nanobdella aerobiophila DSM 111728 (+) 2-3 days | 65 °C Use only 0.50 g/l yeast extract and supplement medium with 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust the pH of the final medium to 2.5. DSMZ BacDive
Archaeon Picrophilus oshimae DSM 9789 55 °C Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. DSMZ BacDive
Archaeon Picrophilus oshimae DSM 9790 55 °C Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. DSMZ BacDive
Archaeon Saccharolobus shibatae DSM 5389 75 °C Adjust pH of medium to 3.0 - 3.5. DSMZ BacDive
Archaeon Sulfolobus acidocaldarius DSM 639 70 °C DSMZ BacDive
Archaeon Sulfuracidifex metallicus DSM 6482 65 °C Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. DSMZ BacDive
Archaeon Sulfuracidifex tepidarius DSM 104736 3-7 days | 65 °C Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. DSMZ BacDive
Archaeon Sulfurisphaera ohwakuensis DSM 12421 75 °C Adjust pH of medium to 3.0 - 3.5. DSMZ BacDive
Archaeon Sulfurisphaera tokodaii DSM 16993 75 °C Supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. DSMZ BacDive
Bacterium Thermobacillus composti DSM 18247 50 °C Modified, with 0.5% yeast extract; solidified by 0.7% Gelrite; pH:9.0 DSMZ BacDive
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