Archaeon |
Acidianus sp. DSM 29038
|
2-3 days | 70 °C
|
Supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5.
|
DSMZ
BacDive
|
Archaeon |
Acidianus sp. DSM 29099
|
3-7 days | 65 °C
|
Use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 - 3.0.
|
DSMZ
BacDive
|
Archaeon |
Acidianus sulfidivorans DSM 18786
|
70 °C
|
Use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150°C overnight. Adjust the pH of the medium to 0.8.
|
DSMZ
BacDive
|
Bacterium |
Laceyella sediminis DSM 45263
|
55 °C | 7.50 (pH) | aerobic
|
Adjust pH of medium to 7.5
|
DSMZ
BacDive
|
Archaeon |
Metallosphaera hakonensis DSM 7519
|
3-7 days | 65 °C
|
Adjust pH of medium to 3.0 - 3.5.
|
DSMZ
BacDive
|
Archaeon |
Metallosphaera javensis DSM 112778
|
(+)
2-3 days | 70 °C
|
Supplement medium with 0.50 g/l powdered sulfur
and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. Adjust the pH of the medium to 3.0.
|
DSMZ
BacDive
|
Archaeon |
Metallosphaera prunae DSM 10039
|
75 °C
|
Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
|
DSMZ
BacDive
|
Archaeon |
Metallosphaera sedula DSM 5348
|
3-7 days | 65 °C
|
Omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium.
|
DSMZ
BacDive
|
Archaeon |
Nanobdella aerobiophila DSM 111728
|
(+)
2-3 days | 65 °C
|
Use only 0.50 g/l yeast extract and supplement medium with 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust the pH of the final medium to 2.5.
|
DSMZ
BacDive
|
Archaeon |
Picrophilus oshimae DSM 9789
|
55 °C
|
Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts.
|
DSMZ
BacDive
|
Archaeon |
Picrophilus oshimae DSM 9790
|
55 °C
|
Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts.
|
DSMZ
BacDive
|
Archaeon |
Saccharolobus shibatae DSM 5389
|
75 °C
|
Adjust pH of medium to 3.0 - 3.5.
|
DSMZ
BacDive
|
Archaeon |
Sulfolobus acidocaldarius DSM 639
|
70 °C
|
|
DSMZ
BacDive
|
Archaeon |
Sulfuracidifex metallicus DSM 6482
|
65 °C
|
Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
|
DSMZ
BacDive
|
Archaeon |
Sulfuracidifex tepidarius DSM 104736
|
3-7 days | 65 °C
|
Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
|
DSMZ
BacDive
|
Archaeon |
Sulfurisphaera ohwakuensis DSM 12421
|
75 °C
|
Adjust pH of medium to 3.0 - 3.5.
|
DSMZ
BacDive
|
Archaeon |
Sulfurisphaera tokodaii DSM 16993
|
75 °C
|
Supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0.
|
DSMZ
BacDive
|
Bacterium |
Thermobacillus composti DSM 18247
|
50 °C
|
Modified, with 0.5% yeast extract; solidified by 0.7% Gelrite; pH:9.0
|
DSMZ
BacDive
|