Modified Wolfe's Mineral Elixir
Internal Solution-ID: S6340
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NTA | 1.500 | g | 1.5 | 7.848 | |
| MgCl2 x 7 H2O | 3.000 | g | 3 | 31.509 | |
| MnSO4 x H2O | 0.500 | g | 0.5 | 2.958 | |
| NaCl | 1.000 | g | 1 | 17.112 | |
| FeSO4 x 7 H2O | 0.100 | g | 0.1 | 0.36 | |
| CoCl2 x 6 H2O | 0.100 | g | 0.1 | 0.77 | |
| CaCl2 x 2 H2O | 0.100 | g | 0.1 | 0.68 | |
| ZnSO4 x 7 H2O | 0.100 | g | 0.1 | 0.348 | |
| CuSO4 x 5 H2O | 0.025 | g | 0.025 | 0.1 | |
| AlK(SO4)2 x 12 H2O | 0.010 | g | 0.01 | 0.021 | |
| H3BO3 | 0.010 | g | 0.01 | 0.162 | |
| Na2MoO4 x 2 H2O | 0.400 | g | 0.4 | 1.653 | |
| NiCl2 x 6 H2O | 0.010 | g | 0.01 | 0.077 | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Add NTA (caution – carcinogenic!) to 500 mL distilled water. | |||||
| 2 Adjust pH to 6.5 with saturated KOH with constant stirring. NTA will go into solution as pH rises. | |||||
| 3 Once the NTA is completely dissolved and the pH is at 6.5, add each mineral salt in order given, letting each dissolve before adding the next. | |||||
| 4 Bring volume up to 1 L with distilled water. | |||||
| 5 Autoclave at 121 °C for 15 min. | |||||
| 6 Store at 4°C. | |||||