Propagation of DSM 25223 in liquid cultures
Internal Solution-ID: S3003
| 1 Add 3 ml of a Caulobacter vibrioides DSM 25117 culture (grown for 24 h in DSMZ medium 1462) to 20 ml of medium and ioculate with 1 ml of a liquid culture of DSM 25223 or transfer some material with an inoculation loop from the lysis zone of a double layer agar plate. Incubate with shaking (ca. 150 rpm) for approximately 48 h. |