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Mail sol. C9

Internal Solution-ID: S6000

Compound Amount Unit Conc. [g/L] Conc. [mM]
Tricine 0.50 g 0.5 2.791
NaCl 35.00 g 35 598.905
Extra salts 3.75 ml - -
Vitamin solution 2.50 ml - -
SE1 (Soil Extract 1) 25.00 ml - -
1 Make up to 1 Litre Filtered natural seawater. Adjust pH to 7.6 – 7.8 with 1M NaOH or 1M HCl, prior to autoclaving. Autoclave at 15 psi for 15 minutes
2 Alternatively, use: Distilled water to 1 Litre and “Ultramarine Synthetica” sea salts 33.60g
C9 2ASW