Mail sol. C5
Internal Solution-ID: S5996
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tricine | 0.50 | g | 0.5 | 2.791 | |
| Extra salts | 3.75 | ml | - | - | |
| Vitamin solution | 2.50 | ml | - | - | |
| SE1 (Soil Extract 1) | 25.00 | ml | - | - | |
|
Natural sea water
(filtered) |
1000.00 | ml | - | - | |
| 1 Make up to 1 Litre with filtered natural seawater. Adjust pH to 7.6 – 7.8 with 1M NaOH or 1M HCl. Add approximately 1 grain of barley to each 25ml of prepared medium prior to autoclaving. Autoclave at 15 psi for 15 minutes. | |||||
| 2 Alternatively, use: Distilled water to 1 Litre and “Ultramarine Synthetica” sea salts 33.60g | |||||
Media with this solution
| C5 | ASW + barley |