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Mail sol. C1

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Internal Solution-ID: S5992

Compound Amount Unit Conc. [g/L] Conc. [mM]
(NH4)2SO4 0.25 g 0.25 1.892
NaNO3
(75 g/l stock solution) 		10.00 ml - -
CaCl2 x 2 H2O
(2.5 g/l stock solution) 		10.00 ml - -
MgSO4 x 7 H2O
(7.5 g/l stock solution) 		10.00 ml - -
K2HPO4 x 3 H2O
(7.5 g/l stock solution) 		10.00 ml - -
KH2PO4
(17.5 g/l stock solution) 		10.00 ml 23.4 171.951
NaCl
(2.5 g/l stock solution) 		10.00 ml - -
Trace Elements (PIV) 6.00 ml - -
1 Once elements are dissolved autoclave at 15 psi for 15 minutes.
Vitamin B1 solution 1.00 ml - -
Vitamin B12 solution 1.00 ml - -
2 The stock solutions are those for 3N-BBM+V. Make up to 1 litre with distilled water and adjust the pH to 3.0 with 1M NaOH or 1M HCl. Autoclave at 15 psi for 15 minutes.
C1 ABM