Main sol. 450
Internal Solution-ID: S5615
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 10 x M9 salts (per l) | 100 | ml | - | - | |
|
MgSO4
(1 M) |
1 | ml | 0.12 | 1 | |
|
CaCl2
(0.1 M) |
1 | ml | 0.011 | 0.1 | |
|
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M) |
1 | ml | 0.373 | 1 | |
|
Glucose
(20%) |
10 | ml | 2 | 11.101 | |
| Proline | 20 | mg | 0.02 | 0.174 | |
| Arginine | 20 | mg/l | 0.02 | 0.115 | |
| Distilled water | 900 | ml | - | - | |
| 1 Adjust pH to 7.4. | |||||
| 2 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. | |||||
Media with this solution
| 450 | SUPPLEMENTED (ARGININE) M9 MEDIUM |