Main sol. J981
Internal Solution-ID: S5010
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.20 | g | 0.189 | 1.392 | |
| NH4Cl | 0.45 | g | 0.426 | 7.967 | |
| NaCl | 1.70 | g | 1.61 | 27.547 | |
| KCl | 0.50 | g | 0.473 | 6.351 | |
| MgCl2 x 6 H2O | 0.40 | g | 0.379 | 1.863 | |
| CaCl2 x 2 H2O | 0.16 | g | 0.152 | 1.031 | |
| Na2SO4 | 4.00 | g | 3.788 | 26.668 | |
| FeCl2 solution | 1.00 | ml | - | - | |
| Trace element solution | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Resazurin | 1.00 | mg | 9.5e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): | |||||
|
NaHCO3
(8.0%) |
30.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Thiamine solution | 1.00 | ml | - | - | |
| Vitamin B12 solution 0.05 g/L | 1.00 | ml | - | - | |
| 2 Distribute the medium into sterile culture vessels (e.g., 50 ml per 120 ml bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with sterile butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere) in the order listed: | |||||
|
Phenol
(freshly prepared, *, 0.1 M) |
10.00 | ml | - | - | |
|
Na2S x 9 H2O
(5%) |
10.00 | ml | - | - | |
Media with this solution
| J981 | ANAEROBIC BICARBONATE-BUFFERED MEDIUM WITH PHENOL |