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Solution A

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Internal Solution-ID: S453

Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.545 4.007
MgCl2 x 6 H2O 0.33 g 0.36 1.77
NaCl 0.40 g 0.436 7.464
NH4Cl 0.40 g 0.436 8.155
CaCl2 x 2 H2O 0.05 g 0.055 0.371
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Clarified rumen fluid 50.00 ml - -
Trypticase peptone
(BD BBL) 		1.00 g 1.091 -
Na2SO4 2.80 g 3.053 21.497
Vitamin K1 solution 1.00 ml - -
Volatile fatty acid mixture 3.10 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.5e-4 0.002
Distilled water 860.00 ml - -
212a TREPONEMA RECTALE MEDIUM