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Main sol. 1610

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Internal Solution-ID: S3352

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (50x salt stock) 20.0 ml - -
Solution 2 (100 mM Fe EDTA) 0.4 ml - -
Solution 3 (100 mM KH2PO4) 10.0 ml - -
Solution 4 (10 mM CuSO4 x 5 H2O) 0.1 ml - -
Solution 5 (30% NaCl) 100.0 ml - -
Solution 6 (100 mM NH4Cl) 20.0 ml - -
Solution 7 (100 mM KNO3) 20.0 ml - -
Solution 8 (100 mM Hepes) 50.0 ml - -
Solution 9 (100µM lanthanides) 1.0 ml - -
Trace element solution 1.0 ml - -
Distilled water 1000.0 ml - -
Methanol
(filter sterilized) 		10.0 ml 7.92 247.176
1 Dilute 20 ml of solution 1 to 700ml with distilled water.
2 Add 100mL of solution 5, 50 mL of solution 8, 20mL of solution 6 and 7 and 10 mL of solution 3.
3 Add 1ml trace element solution.
4 Add 0.4 mL solution 2.
5 Add 0.1 mL solution 4.
6 Add 1.0 mL solution 9.
7 Disolve and bring to 1L.
8 Adjust pH to 7.8 with HCl or NaOH. Autoclave medium.
9 After autoclaving add 10mL per liter of filter sterilized methanol.
1610 METHYLOCEANIBACTER MEDIUM (NaCl-dANMS)