Main sol. 1294
Internal Solution-ID: S2592
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 23.0 | g | 22.908 | 391.998 | |
| KCl | 1.3 | g | 1.295 | 17.369 | |
| KH2PO4 | 0.2 | g | 0.199 | 1.464 | |
| NH4Cl | 0.5 | g | 0.498 | 9.31 | |
| NaNO3 | 0.9 | g | 0.896 | 10.547 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.677 | |
| MgCl2 x 6 H2O | 3.0 | g | 2.988 | 14.698 | |
| Trace element solution SL-10 | 1.0 | ml | - | - | |
| Selenite-tungstate solution | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.0 | g | 0.996 | 9.397 | |
| Wolin's vitamin solution (10x) | 1.0 | ml | - | - | |
| Seven vitamins solution | 1.0 | ml | - | - | |
| Na-pyruvate | 1.1 | g | 1.096 | 9.956 | |
| Na2S x 9 H2O | 0.1 | g | 0.1 | 0.415 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, vitamins, pyruvate and sulfide) and sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, pyruvate and sulfide form anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. Add carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of the complete medium to 6.5 - 6.8, if necessary. | |||||
Media with this solution
| 1294 | DESULFURISPIRILLUM INDICUM MEDIUM |