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Main sol. 1097

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Internal Solution-ID: S2213

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 20.0 g 19.92 340.868
MgCl2 x 6 H2O 3.0 g 2.988 14.698
KCl 0.7 g 0.697 9.352
NH4Cl 0.2 g 0.199 3.724
KH2PO4 0.2 g 0.199 1.464
CaCl2 x 2 H2O 0.2 g 0.199 1.355
Trace element solution SL-10 2.0 ml - -
Selenite-tungstate solution 1.0 ml - -
Sodium resazurin
(0.1% w/v) 		0.5 ml 5.0e-4 0.002
Na2CO3 1.5 g 1.494 14.096
Na2-fumarate 1.6 g 1.594 9.958
Wolin's vitamin solution (10x) 1.0 ml - -
Na2S x 9 H2O 0.3 g 0.299 1.244
Distilled water 1000.0 ml - -
1 Dissolve ingredients (except carbonate, fumarate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fumarate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Stock solutions of vitamins and fumarate should be sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.
1097 SULFUROSPIRILLUM MV MEDIUM