Main sol. 968b
Internal Solution-ID: S1993
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.10 | g | 0.1 | 0.755 | |
| MgSO4 | 25.00 | mg | 0.025 | 0.207 | |
| NaCl | 60.00 | g | 59.821 | 1023.62 | |
| K2HPO4 | 0.15 | g | 0.15 | 0.859 | |
| KH2PO4 | 0.08 | g | 0.08 | 0.586 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 10.60 | g | 10.568 | 99.712 | |
| NaHCO3 | 4.20 | g | 4.187 | 49.846 | |
| Na2HAsO4 x 7 H2O | 3.10 | g | 3.091 | 9.906 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.82 | g | 0.818 | 3.404 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonates, arsenate, vitamins and sulfide), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add solid carbonate and hydrogencarbonate, adjust pH to 9.8, dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or seruim vials and autoclave. Prior to inoculation, add arsenate, vitamins (sterilized by filtration) and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Adjust pH of complete medium to 9.8, if necessary. | |||||
Media with this solution
| 968b | MLMS-1 MEDIUM (ARSENATE) |