Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| Na2HPO4 x 2 H2O | 0.42 | g | 0.42 | 2.959 | |
| Na2HPO4 x H2O | 0.18 | g | 0.18 | 1.268 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.492 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| KCl | 0.10 | g | 0.1 | 1.341 | |
| FeSO4 x 7 H2O | 0.04 | g | 0.04 | 0.144 | |
| Wolin's vitamin solution | 10.00 | ml | - | - | |
| Modified Wolin's mineral solution | 10.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Cresol OR Phenol | 1.00 | mM | - | 1 | |
| 1 Dissolve phosphates separately, Adjust pH to 6.5. Add cresol/phenol and vitamin solution after autoclaving. | |||||
| 2 (e.g.: 20 ml/l of a 4.7 g/l phenol-solution) | |||||
Media with this solution
| 738 | BPHD Medium |