MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder NEW Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 690

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1438

Equipment needed: Autoclave Hungate tubes Filter Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
NH4Cl 0.50 g 0.494 9.237
KH2PO4 0.85 g 0.84 6.172
K2HPO4 0.80 g 0.791 4.539
FeSO4 x 7 H2O
(0.1% w/v in 0.1 N H2SO4) 		1.00 ml 9.9e-4 0.004
Modified Wolin's mineral solution 10.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 4.9e-4 0.002
MgSO4 x 7 H2O 0.25 g 0.247 1.002
NaHCO3 3.00 g 2.964 35.288
D-Glucose 2.00 g 1.976 10.97
Wolin's vitamin solution (10x) 1.00 ml - -
Na2S x 9 H2O 0.30 g 0.296 1.234
Distilled water 1000.00 ml - -
1 Dissolve ingredients except magnesium sulfate, bicarbonate, glucose, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add magnesium sulfate, glucose, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and bicarbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. Adjust pH of complete medium to 7.0 - 7.2, if necessary.
690 DMA MEDIUM