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DSMZ

Strain modifications for Medium 838

4 distinct modifications were found.

Omit Na-acetate and Na-ascorbate. Supplement medium after autoclaving with 10.00 ml/l of a Wolin's vitamin solution (see medium 141), 1.00 g/l Na-DL-lactate and 1.00 g/l yeast extract added from sterile anoxic stock solutions prepared under 100% N2 gas.

Modified strains

Desulfitobacterium metallireducens DSM 15288
Desulfitobacterium hafniense DSM 16228

Annotated modifications

1 g Na-(DL)-lactate
Na-acetate
Na-ascorbate
1 g Yeast extract
+ 10 ml Wolin's vitamin solution

Prepare medium without Na-ascorbate.

Modified strains

Geotalea toluenoxydans DSM 19350
Geotalea daltonii DSM 22248
Geomobilimonas luticola DSM 24905
Geobacter sp. DSM 26175

Annotated modifications

Na-ascorbate

Supplement medium with 1.00 g/l NaCl. After autoclaving supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace Na-ascorbate with 0.15 g/ of L-cysteine-HCl x H2O added from a sterile anoxic stock solution prepared under 100% N2 gas.

Modified strains

Geobacter sp. DSM 9736

Annotated modifications

+ 1 ml Seven vitamins solution
Na-ascorbate
1 g NaCl
0.15 g L-Cysteine HCl x H2O

Supplement medium with 20.00 g/l NaCl and omit Na-ascorbate. After autoclaving supplement medium with 10.00 ml/l of a sterile anoxic Wolin's vitamin solution (see medium 141).

Modified strains

Geopsychrobacter electrodiphilus DSM 16401

Annotated modifications

Na-ascorbate
20 g NaCl
+ 10 ml Wolin's vitamin solution
Modifications
Omit Na-acetate and Na-ascorbate. Supplement medium after autoclaving with 10.00 ml/l of a Wolin's vitamin solution (see medium 141), 1.00 g/l Na-DL-lactate and 1.00 g/l yeast extract added from sterile anoxic stock solutions prepared under 100% N2 gas. Prepare medium without Na-ascorbate. Supplement medium with 1.00 g/l NaCl. After autoclaving supplement medium with 1.00 ml/l of seven vitamins solution (see medium 503) and replace Na-ascorbate with 0.15 g/ of L-cysteine-HCl x H2O added from a sterile anoxic stock solution prepared under 100% N2 gas. Supplement medium with 20.00 g/l NaCl and omit Na-ascorbate. After autoclaving supplement medium with 10.00 ml/l of a sterile anoxic Wolin's vitamin solution (see medium 141).