Strain modifications for Medium 641

Replace lactate with 0.2 g/l Na-acetate. After inoculation pressurize culture vessels to 1 bar overpressure with sterile 80% H₂ and 20% CO₂ gas mixture.

Replace lactate with 0.4 g/l Na-benzoate added to the autoclaved medium from a sterile anoxic stock solution.

Replace lactate with 0.9 g/l glycerol and adjust pH of complete medium to 7.5.

Replace lactate with 2.0 g/l D-glucose added to the autoclaved medium from a sterile anoxic stock solution.

Replace lactate with 2.5 g/l Na-pyruvate added to the autoclaved medium from an anoxic stock solution sterilized by filtration and replace sodium sulfide with 0.15 g/l DL-dithiothreitol (DTT) added from an anoxic stock solution sterilized by filtration.

Replace lactate with 2.5 g/l Na-pyruvate added to the autoclaved medium from an anoxic stock solution sterilized by filtration and supplement medium with an additional amount of 1.0 g/l Na₂S₂O₃ x 5 H₂O added from an anoxic stock solution sterilized by filtration.

Replace lactate with 2.5 g/l Na-pyruvate added to the autoclaved medium from an anoxic stock solution sterilized by filtration.

Replace lactate with 3.5 g/l D-fructose added to the autoclaved medium from an anoxic stock solution sterilized by filtration.

Replace lactate with 5.0 ml/l methanol added to the autoclaved medium from an anoxic stock solution prepared under 100% N₂ gas.

Supplement medium with 2.20 g/l pyruvate, 1.20 g/l taurine and 0.20 mg/l 1,4-naphthochinone added to the autoclaved medium from anoxic stock solutions sterilized by filtration,