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DSMZ

Strain modifications for Medium 520

2 distinct modifications were found.

Adjust pH of final medium to 8.0.

Modified strains

Anaerobacterium chartisolvens DSM 27016

Annotated modifications

8.0

Use 5.00 g/l D-glucose as the substrate. Supplement medium after autoclaving with 0.50 g/l Na2S x 9 H2O added from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of complete medium to 6.8 - 7.0, if necessary.

Modified strains

Clostridium thermopalmarium DSM 5974
Thermoanaerobacter brockii subsp. lactiethylicus DSM 9801
Gracilibacter thermotolerans DSM 17427

Annotated modifications

6.8 - 7.0
0.5 g Na2S x 9 H2O
5 g D-Glucose
Modifications
Adjust pH of final medium to 8.0. Use 5.00 g/l D-glucose as the substrate. Supplement medium after autoclaving with 0.50 g/l Na2S x 9 H2O added from a sterile anoxic stock solution prepared under 100% N2 gas and adjust pH of complete medium to 6.8 - 7.0, if necessary.