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DSMZ

Strain modifications for Medium 504

6 distinct modifications were found.

Replace glucose with 0.50 g/l of pyrogallol as the substrate. When growth appears feed once more with 0.50 g/l pyrogallol.

Modified strains

Pelobacter massiliensis DSM 6233

Annotated modifications

1.00 g Pyrogallol
to Solution C
D-Glucose
to Solution C

Replace glucose with 10.00 g/l Proteose peptone (BD Difco) as substrate. Adjust pH of complete medium to 6.5 - 7.0.

Modified strains

Tepidibacter thalassicus DSM 15285
Clostridium tepidiprofundi DSM 19306

Annotated modifications

6.5 - 7.0
- Solution C
10.00 g Proteose peptone (BD Difco)
to Solution A

Replace glucose with 2.50 g/l maltose and 2.00 g/l yeast extract as substrates.

Modified strains

Psychrilyobacter atlanticus DSM 19335
Psychrilyobacter sp. DSM 17953

Annotated modifications

2.00 g Yeast extract
to Solution A
D-Glucose
to Solution C
2.50 g D-Maltose
to Solution C

Replace glucose with 2.50 g/l of Na2-succinate and 0.50 g/l yeast extract as substrates.

Modified strains

DSM 5848
Propionigenium maris DSM 9537

Annotated modifications

0.50 g Yeast extract
to Solution A
2.50 g Sodium succinate
to Solution C
D-Glucose
to Solution C

Supplement medium with 0.50 g/l yeast extract.

Modified strains

Anaerophaga thermohalophila DSM 12881
Thermophagus xiamenensis DSM 19012
Anaerophaga sp. DSM 25728

Annotated modifications

0.50 g Yeast extract
to Solution A

Use 0.30 g/l L-Cysteine-HCl x H2O and 1.50 g/l acetaldehyde as substrates. Acetaldehyde should be added in aliquots of 0.20 g/l at regular intervals until full growth is achieved.

Modified strains

Syntrophotalea acetylenivorans DSM 106009

Annotated modifications

0.30 g L-Cysteine HCl x H2O
to Solution A
D-Glucose
to Solution C
1.50 g Acetaldehyde
to Solution C
Modifications
Replace glucose with 0.50 g/l of pyrogallol as the substrate. When growth appears feed once more with 0.50 g/l pyrogallol. Replace glucose with 10.00 g/l Proteose peptone (BD Difco) as substrate. Adjust pH of complete medium to 6.5 - 7.0. Replace glucose with 2.50 g/l maltose and 2.00 g/l yeast extract as substrates. Replace glucose with 2.50 g/l of Na2-succinate and 0.50 g/l yeast extract as substrates. Supplement medium with 0.50 g/l yeast extract. Use 0.30 g/l L-Cysteine-HCl x H2O and 1.50 g/l acetaldehyde as substrates. Acetaldehyde should be added in aliquots of 0.20 g/l at regular intervals until full growth is achieved.