Strain modifications for Medium 381
30 distinct modifications were found.
Adjust pH of final medium to 6.0.
Modified strains
Annotated modifications
| 6.0 |
Add 100g NaCl (10%) for 1000 ml medium.
Add to medium Na-sesquicarbonate solution (1 mL in 10 mL) to achieve pH 9.5 plus CaCl2. Na-sesquicarbonate solution: NaHCO3 4.2 g, Na2CO3 anh. 5.3 g, dist. water 100 mL
Agar plates with 1% hexadecane. Alternatively, place a drop of hexadecane on the lid of the petridish; Use 3 ml medium 1 or medium 381 for rehydration of freeze dried culture.
If used as phage host, growth at 32°C recommended
Medium for chitin decomposition see reference (9297).
Or any conventional minimal medium supplemented with 0.2% glucose and thiamine
Plus 0,35% Tween 80
Plus 10 mg/L MnSO4
Plus 100 mM boric acid
Plus 2% NaCl
Plus 3% NaCl
Plus 3% NaCl (final concentration)
Plus ampicillin 100 mg/ml
Plus Kanamycin 50 mg/ml
Plus 0.2% glucose
plus 10% NaCl
Modified strains
plus 20g/l NaCl
Modified strains
Annotated modifications
| 20 g/l NaCl |
plus 3% NaCl (final concentration)
Plus 50 mg/L Nalidixic acid
Rehydrate and grow cells from the ampoule in liquid medium. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells in liquid broth 381. Subsequent subculturing may be carried out in liquid medium or on agar plates
Resuspend and grow freeze-dried cells from ampoules in liquid medium first. Subsequently, cultivation may be carried out in liquid medium and on agar plates.
Solified with 15 g/l agar
With only 5g/L NaCl and supplemented with 50 µg/mL 5-aminolevulinic acid (ALA)