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DSMZ

Strain modifications for Medium 269

4 distinct modifications were found.

Reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5.

Modified strains

Acidocella aminolytica DSM 11237
Acidobacterium capsulatum DSM 11244

Annotated modifications

3.5
Yeast extract
0.1 g Yeast extract

Replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of complete medium to 2.5.

Modified strains

Acidiphilium sp. DSM 100189

Annotated modifications

D-Glucose
2.5
7 g Fe SO4 x 7 H2O
1.8 g D-Galactose

Replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5.

Modified strains

Acidiphilium multivorum DSM 11245

Annotated modifications

3.5
Yeast extract
0.1 g Trypticase soy broth

Supplement medium with 1.0 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 4.0.

Modified strains

Acidocella aluminiidurans DSM 108313

Annotated modifications

4.0
1 g Trypticase soy broth
Modifications
Reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5. Replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of complete medium to 2.5. Replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5. Supplement medium with 1.0 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 4.0.