Strain modifications for Medium 220

71 distinct modifications were found.

Supplement medium with 10 ml/l Methanol.

Modified strains

Methylobacillus sp. DSM 5746

Annotated modifications

10 ml/l Methanol

Adjust pH of final medium to 8.5 - 9.0.

Modified strains

Amphibacillus sediminis DSM 21624

Annotated modifications

8.5 - 9.0

Adjust pH of final medium to 7.0.

Modified strains

Cytobacillus kochii DSM 23667

Annotated modifications

7.0

Adjust pH of final medium to 8.0.

Modified strains

Amphibacillus cookii DSM 23721
Robertmurraya kyonggiensis DSM 26768

Annotated modifications

8.0

Adjust pH of final medium to 9.0.

Modified strains

Amphibacillus jilinensis DSM 24783
Halalkalibacter nanhaiisediminis DSM 27953
Amphibacillus marinus DSM 27991
Alteribacter populi DSM 104632
Halalkalibacter urbisdiaboli DSM 104651
Bacillus alkalisoli DSM 104056
Bacillus solitudinis DSM 104631
Isoptericola sp. DSM 21904

Annotated modifications

9.0

Adjust pH of final medium to 10.0.

Modified strains

Amphibacillus indicireducens DSM 27992

Annotated modifications

10.0

(liquid medium)

Modified strains

Thauera sp. DSM 6505

Annotated modifications

Agar

(shaken liquid cultures to reactivate the lyophilisate)

Modified strains

Mycobacterium gilvum DSM 9487

Annotated modifications

Agar

adjust pH with a Na-sesquicarbonate solution

Modified strains

Thalassorhabdus sp. DSM 104055
Alkalihalobacillus sp. DSM 104053

adjust pH with Na-sesquicarbonate solution

Modified strains

Alkalihalobacillus sp. DSM 104054

Adjust pH with Na-sesquicarbonate solution; pH:9.0

Modified strains

Alkalihalobacillus trypoxylicola DSM 25300

Annotated modifications

9.0

Adjust to pH 9.5 with Na-sesquicarbonate solution

Modified strains

Amphibacillus tropicus DSM 13870

Annotated modifications

9.5

Plus 0.01M Na-tetraborate

Modified strains

Lysinibacillus boronitolerans DSM 17140

Annotated modifications

0.01 M Na-Tetraborate

Plus 0.1% urea

Modified strains

Lederbergia lenta DSM 9
Paenibacillus ripae DSM 104672

Annotated modifications

1.0 g/l Urea

Plus 0.2% yeast and MnSO4 (use liquid medium without agar for growing the strain)

Modified strains

Geobacillus stromboliensis DSM 15392

Annotated modifications

2.0 g/l Yeast
Agar
2 g/l MnSO4

Plus 0.5% yeast extract

Modified strains

Bacillus thermoterrestis DSM 12833

Annotated modifications

5.0 g/l Yeast extract

Plus 0.7% xylan plus 0.4% yeast plus 10 µg/ml MnSO4

Modified strains

Paenibacillus phyllosphaerae DSM 17399

Annotated modifications

7.0 g/l Xylan
4.0 g/l Yeast
10 mg/l MnSO4

Plus 1% methanol

Modified strains

Methylorubrum extorquens DSM 13060

Annotated modifications

10.0 g/l Methanol

Plus 1/10 Na-sesquicarbonate solution; (see medium 31)

Modified strains

Halalkalibacter hemicellulosilyticus DSM 16731

Plus 10 % NaCl

Modified strains

Lentibacillus salinarum DSM 21356

Annotated modifications

100.0 g/l NaCl

Plus 10 mg/l MnSO4

Modified strains

Anoxybacillus rupiensis DSM 17127
Paenibacillus uliginis DSM 21861
Shouchella sp. DSM 21911
Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 21923
Paenibacillus hunanensis DSM 22170
Anoxybacillus rupiensis DSM 22226
Paenibacillus hodogayensis DSM 22253
Paenibacillus elgii DSM 22254
Paenibacillus barengoltzii DSM 22255
Paenibacillus provencensis DSM 22280
Paenibacillus urinalis DSM 22281
Paenibacillus tianmuensis DSM 22342
Psychrobacillus psychrotolerans DSM 22887
Psychrobacillus psychrotolerans DSM 22888
Psychrobacillus psychrodurans DSM 22889
Bacillus cytotoxicus DSM 22905
Geobacillus uzenensis DSM 23175
Bacillus sp. DSM 23220
Caldibacillus thermolactis DSM 23332
Oceanobacillus kimchii DSM 23341
Bacillus sp. DSM 23473
Bacillus sp. DSM 23474
Bacillus sp. DSM 23482
Paenibacillus xylanisolvens DSM 25299
Sporosarcina newyorkensis DSM 23966

Annotated modifications

10.0 mg/l MnSO4

Plus 10 mg/l MnSO4; pH 6.8

Modified strains

Lederbergia graminis DSM 22162

Annotated modifications

10.0 mg/l MnSO4
6.8

Plus 10 mg/L MnSO4; pH 7-10

Modified strains

Cytobacillus purgationiresistens DSM 23494

Annotated modifications

10 mg/L MnSO4
7.0

Plus 10 mg/L MnSO4; pH 7.0

Modified strains

Paenibacillus jilunlii DSM 23019
Paenibacillus sophorae DSM 23020

Annotated modifications

10 mg/L MnSO4
7.0

Plus 10 mg/l MnSO4; pH 7.2

Modified strains

Bacillus sp. DSM 21957
Sporosarcina thermotolerans DSM 22203
Sporosarcina contaminans DSM 22204
Fictibacillus sp. DSM 22833
Virgibacillus soli DSM 22952
Ornithinibacillus contaminans DSM 22953
Paenibacillus purispatii DSM 22991
Sporosarcina newyorkensis DSM 23541
Sporosarcina newyorkensis DSM 23543

Annotated modifications

10.0 mg/l MnSO4
7.2

Plus 10 mg/L MnSO4; pH 7.3

Modified strains

Paenibacillus faecis DSM 23593

Annotated modifications

10 mg/L MnSO4
7.3

Plus 10 mg/L MnSO4; pH 8.0

Modified strains

Anoxybacillus tengchongensis DSM 23211
Anoxybacillus eryuanensis DSM 23212

Annotated modifications

10 mg/L MnSO4
8.0

Plus 10 µg/ml MnSO4

Modified strains

Paenibacillus thailandensis DSM 22866
Saccharibacillus kuerlensis DSM 22868
Paenibacillus sputi DSM 22699
Heyndrickxia acidiproducens DSM 23148
Paenibacillus macquariensis subsp. defensor DSM 23149
Oceanobacillus sojae DSM 23151

Annotated modifications

10 µg/ml MnSO4

Plus 10 µg/ml MnSO4; pH 7.2

Modified strains

Paenibacillus residui DSM 22072
Paenibacillus residui DSM 22073

Annotated modifications

10 µg/ml MnSO4
7.2

Plus 10% NaCl and MnSO4, pH 7.5

Modified strains

Oceanobacillus sp. DSM 15766
Oceanobacillus sp. DSM 15767

Annotated modifications

100.0 g/l NaCl
10 % MnSO4
7.5

Plus 10% NaCl; pH 9

Modified strains

Alkalibacillus flavidus DSM 23520

Annotated modifications

100.0 g/l NaCl
9.0

Plus 100 g/L sodium sesquicarbonate plus 10 mg/L MnSO4; ca pH 9

Modified strains

Halalkalibacter alkalisediminis DSM 22382

Annotated modifications

10 mg/L MnSO4
100 g/l Sodium sesquicarbonate
9.0

Plus 10mg/l MnSO 4

Modified strains

Paenibacillus tundrae DSM 21291

Annotated modifications

10 mg/l MnSO4

Plus 10mg/l MnSO4

Modified strains

Paenibacillus xylanexedens DSM 21292
Paenibacillus sp. DSM 23624

Annotated modifications

10.0 mg/l MnSO4

Plus 10mg/L MnSO4; pH 7.0

Modified strains

Cytobacillus horneckiae DSM 23495

Annotated modifications

10 mg/L MnSO4
7.0

Plus 10mg/L MnSO4; pH 7.2

Modified strains

Sporosarcina newyorkensis DSM 23540
Sporosarcina newyorkensis DSM 23542
Sporosarcina newyorkensis DSM 23544

Annotated modifications

10 mg/L MnSO4
7.2

Plus 10µg/ml MnSO4

Modified strains

Paenibacillus massiliensis subsp. massiliensis DSM 16942
Paenibacillus timonensis DSM 16943
Paenibacillus cookii DSM 16944
Paenibacillus cineris DSM 16945
Paenibacillus favisporus DSM 17253
Paenibacillus rhizosphaerae DSM 17254
Paenibacillus xylanilyticus DSM 17255

Annotated modifications

10 mg/l MnSO4

Plus 16% NaCl and 10 mg/l MnSO4; pH 9

Modified strains

Alkalicoccus saliphilus DSM 15402

Annotated modifications

160.0 g/l NaCl
10 mg/l MnSO4
9.0

Plus 2 % urea

Modified strains

Sporosarcina sp. DSM 23526

Annotated modifications

20.0 g/l Urea

Plus 2% NaCl

Modified strains

Virgibacillus halophilus DSM 21623

Annotated modifications

20.0 g/l NaCl

Plus 2.5% NaCl plus 0.05 M H3BO3 plus 10 µg/ml MnSO4

Modified strains

Mesobacillus boroniphilus DSM 17376

Annotated modifications

10 mg/l MnSO4
0.05 M H3BO3
25.0 g/l NaCl

Plus 5% NaCl

Modified strains

Virgibacillus sp. DSM 24891

Annotated modifications

50.0 g/l NaCl

Plus 5% NaCl; pH 9.0

Modified strains

Alkalicoccus daliensis DSM 26767

Annotated modifications

50.0 g/l NaCl
9.0

Plus 50% soil extract

Modified strains

Ureibacillus terrenus DSM 12654
Ureibacillus thermosphaericus DSM 12655
Ureibacillus terrenus DSM 12656

Annotated modifications

50 % Soil extract

Plus 50% soil extract (see medium 12)

Modified strains

Brevibacillus limnophilus DSM 6472

Annotated modifications

500.0 g/l Soil extract

Plus 8% NaCl

Modified strains

Bacillus sp. DSM 16818

Annotated modifications

80.0 g/l NaCl

Plus Manganese sulfate (10 µg/ml)

Modified strains

Paenibacillus glycanilyticus DSM 17608

Annotated modifications

10 mg/l Manganese Sulfate

Plus MnSO4 (10µg/ml), 0.05M H3BO3, 2.5 % NaCl

Modified strains

Gracilibacillus boraciitolerans DSM 17256

Annotated modifications

10 mg/l MnSO4
0.05 M H3BO3
2.5 % NaCl

Plus Na-sesquicarbonate solution (see medium 31); pH 10

Modified strains

Paenibacillus campinasensis DSM 21989

Annotated modifications

10.0

Plus soil extract 50%, see medium 12

Modified strains

Paenibacillus sp. DSM 21009

Annotated modifications

50 % Soil extract

Plus urea (20g/l)

Modified strains

Sporosarcina pasteurii DSM 33

Annotated modifications

20 g/l Urea

Plus 10 mg/l MnSO4

Modified strains

Geobacillus sp. DSM 14791
Geobacillus sp. DSM 22570

Annotated modifications

10.0 mg/l MnSO4

plus 10 µg/ml MnSO4

Modified strains

Annotated modifications

10 mg/l MnSO4

plus 10% sodium sesquicarbonate

Modified strains

Geomicrobium sediminis DSM 23309

Annotated modifications

10 % Sodium sesquicarbonate

plus 2.5% NaCl plus 0.05M H3BO3 plus 10µg/ml MnSO4

Modified strains

Annotated modifications

2.5 % NaCl
0.05 M H3BO3
10 mg/l MnSO4

plus 5% NaCl

Modified strains

plus NaCl 50 g/L

Modified strains

Tetragenococcus muriaticus DSM 15685

Prepared in 1/10 strength plus MnSO4

Modified strains

Anoxybacillus kamchatkensis DSM 14988

Rehydrate and grow freeze dried cells from the ampoule in liquid medium 220. Subsequent subculturing may be carried out in liquid medium or on agar 220, or in medium 465.

Modified strains

Comamonas testosteroni DSM 11414

Rehydrate and grow freeze-dried cells from ampoule in 5 ml liquid medium. Subsequently, culturing may be carried out on agar plates and in medium 457 with substrate

Modified strains

Paraburkholderia hospita DSM 7336

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates

Modified strains

Pseudomonas fluorescens DSM 7355

Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates and in medium 465 plus gentisinic acid

Modified strains

Pseudaminobacter salicylatoxidans DSM 7005

Rehydrate and grow lyophilized cels from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates.

Modified strains

Cellvibrio ostraviensis DSM 16616

Tenfold diluted (1/10) plus 10 µg/ml MnSO4

Modified strains

Paenibacillus pueri DSM 22870

Annotated modifications

10 µg/ml MnSO4

Two-fold diluted

Modified strains

Paenibacillus alkaliterrae DSM 17040

With 10 mg/l MnSO4

Modified strains

Aneurinibacillus thermoaerophilus DSM 6379
Aneurinibacillus thermoaerophilus DSM 6459
Aneurinibacillus thermoaerophilus DSM 6460
Anoxybacillus flavithermus subsp. flavithermus DSM 21510
Anoxybacillus kaynarcensis DSM 21706
Paenibacillus sp. DSM 21746
Aneurinibacillus thermoaerophilus DSM 6458

Annotated modifications

10.0 mg/l MnSO4

With 10% NaCl

Modified strains

Virgibacillus salexigens DSM 11483

Annotated modifications

100.0 g/l NaCl

With 100 g/L NaCl; pH 8.0

Modified strains

Shouchella shacheensis DSM 26902

Annotated modifications

100 g/L NaCl
8.0

With 34 g/l NaCl

Modified strains

Halomonas elongata DSM 6706

Annotated modifications

34.0 g/l NaCl

With 5% yeast extract

Modified strains

Geobacillus sp. DSM 13240

Annotated modifications

50.0 g/l Yeast extract

Plus 10 mg/l MnSO4; pH 10

Modified strains

Annotated modifications

10.0
Modifications
Supplement medium with 10 ml/l Methanol. Adjust pH of final medium to 8.5 - 9.0. Adjust pH of final medium to 7.0. Adjust pH of final medium to 8.0. Adjust pH of final medium to 9.0. Adjust pH of final medium to 10.0. (liquid medium) (shaken liquid cultures to reactivate the lyophilisate) adjust pH with a Na-sesquicarbonate solution adjust pH with Na-sesquicarbonate solution Adjust pH with Na-sesquicarbonate solution; pH:9.0 Adjust to pH 9.5 with Na-sesquicarbonate solution Plus 0.01M Na-tetraborate Plus 0.1% urea Plus 0.2% yeast and MnSO4 (use liquid medium without agar for growing the strain) Plus 0.5% yeast extract Plus 0.7% xylan plus 0.4% yeast plus 10 µg/ml MnSO4 Plus 1% methanol Plus 1/10 Na-sesquicarbonate solution; (see medium 31) Plus 10 % NaCl Plus 10 mg/l MnSO4 Plus 10 mg/l MnSO4; pH 6.8 Plus 10 mg/L MnSO4; pH 7-10 Plus 10 mg/L MnSO4; pH 7.0 Plus 10 mg/l MnSO4; pH 7.2 Plus 10 mg/L MnSO4; pH 7.3 Plus 10 mg/L MnSO4; pH 8.0 Plus 10 µg/ml MnSO4 Plus 10 µg/ml MnSO4; pH 7.2 Plus 10% NaCl and MnSO4, pH 7.5 Plus 10% NaCl; pH 9 Plus 100 g/L sodium sesquicarbonate plus 10 mg/L MnSO4; ca pH 9 Plus 10mg/l MnSO 4 Plus 10mg/l MnSO4 Plus 10mg/L MnSO4; pH 7.0 Plus 10mg/L MnSO4; pH 7.2 Plus 10µg/ml MnSO4 Plus 16% NaCl and 10 mg/l MnSO4; pH 9 Plus 2 % urea Plus 2% NaCl Plus 2.5% NaCl plus 0.05 M H3BO3 plus 10 µg/ml MnSO4 Plus 5% NaCl Plus 5% NaCl; pH 9.0 Plus 50% soil extract Plus 50% soil extract (see medium 12) Plus 8% NaCl Plus Manganese sulfate (10 µg/ml) Plus MnSO4 (10µg/ml), 0.05M H3BO3, 2.5 % NaCl Plus Na-sesquicarbonate solution (see medium 31); pH 10 Plus soil extract 50%, see medium 12 Plus urea (20g/l) Plus 10 mg/l MnSO4 plus 10 µg/ml MnSO4 plus 10% sodium sesquicarbonate plus 2.5% NaCl plus 0.05M H3BO3 plus 10µg/ml MnSO4 plus 5% NaCl plus NaCl 50 g/L Prepared in 1/10 strength plus MnSO4 Rehydrate and grow freeze dried cells from the ampoule in liquid medium 220. Subsequent subculturing may be carried out in liquid medium or on agar 220, or in medium 465. Rehydrate and grow freeze-dried cells from ampoule in 5 ml liquid medium. Subsequently, culturing may be carried out on agar plates and in medium 457 with substrate Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates and in medium 465 plus gentisinic acid Rehydrate and grow lyophilized cels from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates. Tenfold diluted (1/10) plus 10 µg/ml MnSO4 Two-fold diluted With 10 mg/l MnSO4 With 10% NaCl With 100 g/L NaCl; pH 8.0 With 34 g/l NaCl With 5% yeast extract Plus 10 mg/l MnSO4; pH 10