Strain modifications for Medium 220
71 distinct modifications were found.
Supplement medium with 10 ml/l Methanol.
Adjust pH of final medium to 8.5 - 9.0.
Adjust pH of final medium to 7.0.
Adjust pH of final medium to 8.0.
Adjust pH of final medium to 9.0.
Modified strains
Annotated modifications
| 9.0 |
Adjust pH of final medium to 10.0.
(shaken liquid cultures to reactivate the lyophilisate)
adjust pH with a Na-sesquicarbonate solution
Adjust pH with Na-sesquicarbonate solution; pH:9.0
Adjust to pH 9.5 with Na-sesquicarbonate solution
Plus 0.01M Na-tetraborate
Plus 0.1% urea
Plus 0.2% yeast and MnSO4 (use liquid medium without agar for growing the strain)
Plus 0.5% yeast extract
Plus 0.7% xylan plus 0.4% yeast plus 10 µg/ml MnSO4
Plus 1% methanol
Plus 1/10 Na-sesquicarbonate solution; (see medium 31)
Plus 10 % NaCl
Plus 10 mg/l MnSO4
Modified strains
Annotated modifications
| 10.0 mg/l MnSO4 |
Plus 10 mg/l MnSO4; pH 6.8
Plus 10 mg/L MnSO4; pH 7-10
Plus 10 mg/L MnSO4; pH 7.0
Plus 10 mg/l MnSO4; pH 7.2
Modified strains
Annotated modifications
| 10.0 mg/l MnSO4 | |
| 7.2 |
Plus 10 mg/L MnSO4; pH 7.3
Plus 10 mg/L MnSO4; pH 8.0
Plus 10 µg/ml MnSO4
Plus 10 µg/ml MnSO4; pH 7.2
Plus 10% NaCl and MnSO4, pH 7.5
Plus 10% NaCl; pH 9
Plus 100 g/L sodium sesquicarbonate plus 10 mg/L MnSO4; ca pH 9
Plus 10mg/l MnSO 4
Plus 10mg/l MnSO4
Plus 10mg/L MnSO4; pH 7.0
Plus 10mg/L MnSO4; pH 7.2
Plus 10µg/ml MnSO4
Modified strains
Annotated modifications
| 10 mg/l MnSO4 |
Plus 16% NaCl and 10 mg/l MnSO4; pH 9
Plus 2% NaCl
Plus 2.5% NaCl plus 0.05 M H3BO3 plus 10 µg/ml MnSO4
Plus 5% NaCl; pH 9.0
Plus 50% soil extract
Plus 50% soil extract (see medium 12)
Plus Manganese sulfate (10 µg/ml)
Plus MnSO4 (10µg/ml), 0.05M H3BO3, 2.5 % NaCl
Plus Na-sesquicarbonate solution (see medium 31); pH 10
Plus soil extract 50%, see medium 12
Plus urea (20g/l)
Plus 10 mg/l MnSO4
plus 10 µg/ml MnSO4
Modified strains
Annotated modifications
| 10 mg/l MnSO4 |
plus 10% sodium sesquicarbonate
plus 2.5% NaCl plus 0.05M H3BO3 plus 10µg/ml MnSO4
Modified strains
Annotated modifications
| 2.5 % NaCl | |
| 0.05 M H3BO3 | |
| 10 mg/l MnSO4 |
plus 5% NaCl
Modified strains
Rehydrate and grow freeze dried cells from the ampoule in liquid medium 220. Subsequent subculturing may be carried out in liquid medium or on agar 220, or in medium 465.
Rehydrate and grow freeze-dried cells from ampoule in 5 ml liquid medium. Subsequently, culturing may be carried out on agar plates and in medium 457 with substrate
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates
Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 220. Subsequent subculturing may be carried out in liquid medium or on agar plates and in medium 465 plus gentisinic acid
Rehydrate and grow lyophilized cels from the ampoule in 5 ml liquid broth. Subsequent subculturing may be carried out in liquid medium or on agar plates.
Tenfold diluted (1/10) plus 10 µg/ml MnSO4
With 10 mg/l MnSO4
Modified strains
Annotated modifications
| 10.0 mg/l MnSO4 |
With 10% NaCl
With 100 g/L NaCl; pH 8.0
With 5% yeast extract
Plus 10 mg/l MnSO4; pH 10
Modified strains
Annotated modifications
| 10.0 |