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DSMZ

Strain modifications for Medium 120a

3 distinct modifications were found.

Modified strains

Increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 - 7.0 and add only 5.00 ml/l methanol.

Modified strains

Methanosarcina lacustris DSM 13486
Methanosarcina lacustris DSM 10334

Annotated modifications

NaHCO3
Methanol
6.8 - 7.0
5.00 ml Methanol
2.00 g NaHCO3

Increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 - 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure.

Modified strains

Methanosarcina mazei DSM 4556

Annotated modifications

NaHCO3
Methanol
6.8 - 7.0
80% H2, 20% CO2
5.00 ml Methanol
2.00 g NaHCO3
Modifications
Increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 - 7.0 and add only 5.00 ml/l methanol. Increase amount of NaHCO3 to 2.00 g/l to achieve a pH of 6.8 - 7.0 and add only 5.00 ml/l methanol. After inoculation pressurize vials with sterile 80% H2 and 20% CO2 gas mixture to 1 bar overpressure.