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DSMZ

Strain modifications for Medium 120

3 distinct modifications were found.

Replace methanol with 5.00 g/l trimethylamine-HCl.

Modified strains

Methanosarcina barkeri DSM 1538
Methanosarcina mazei DSM 7058
Methanosarcina mazei DSM 9195

Annotated modifications

Methanol
5.00 g Trimethylamine-HCl

Supplement medium with 5% (v/v) clarified rumen fluid (see medium 1310) and adjust pH to 6.5 - 6.8.

Modified strains

Methanosarcina thermophila DSM 1825
Methanosarcina thermophila DSM 7081
Methanosarcina sp. DSM 11855

Annotated modifications

6.5 - 6.8
+ 50.0 ml Clarified rumen fluid

Supplement medium with 6.00 g/l NaCl.

Modified strains

Methanosarcina horonobensis DSM 21571

Annotated modifications

6.00 g NaCl
Modifications
Replace methanol with 5.00 g/l trimethylamine-HCl. Supplement medium with 5% (v/v) clarified rumen fluid (see medium 1310) and adjust pH to 6.5 - 6.8. Supplement medium with 6.00 g/l NaCl.