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DSMZ

Strain modifications for Medium 1011c

3 distinct modifications were found.

Omit Na-dithionite solution (5% w/v), Yeast extract, Na2S2O3 x 5 H2O, Na-pyruvate, and Na-lactate. Incubate with a gas phase of 75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar.

Modified strains

Annotated modifications

Yeast extract
Na-pyruvate
Na-lactate
Na2S2O3 x 5 H2O
- Na-dithionite solution (5% w/v)
75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar

Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5.

Modified strains

Thiofractor thiocaminus DSM 22050

Annotated modifications

3 g Sulfur powder
1 g NaNO3
Yeast extract
Na-pyruvate
6.0 - 6.5
Na-lactate

Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate, yeast extract and dithionite. Upon autoclaving add 10.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. After inoculation do not pressurize with 80% H2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 10% of the headspace.

Modified strains

Sulfurovum aggregans DSM 27205

Annotated modifications

10 g Sulfur powder
- Na-dithionite solution (5% w/v)
Yeast extract
Na-pyruvate
6.0 - 6.5
Na-lactate
1 g NaNO3
73% H2, 18% CO2, 7% N2, 2% O2
Modifications
Omit Na-dithionite solution (5% w/v), Yeast extract, Na2S2O3 x 5 H2O, Na-pyruvate, and Na-lactate. Incubate with a gas phase of 75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar. Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate, yeast extract and dithionite. Upon autoclaving add 10.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. After inoculation do not pressurize with 80% H2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 10% of the headspace.