908 .
HALORHODOSPIRA NEUTRIPHILA MEDIUM
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Selected strain: Halorhodospira neutriphila DSM 15116
This strain uses the standard medium without modifications.
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.00 | g | ||
|
|
NH4Cl | 0.50 | g | ||
|
|
NaCl | 100.00 | g | ||
|
|
MgCl2 x 6 H2O | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.05 | g | ||
|
|
Trace element solution SL-12 | 1.00 | ml | ||
|
|
NaHCO3
(10%, w/v) |
20.00 | ml | ||
|
|
Na2S x 9 H2O
(10%, w/v) |
7.50 | ml | ||
|
|
Seven vitamins solution | 1.00 | ml | ||
|
|
Sodium acetate
(0.5 M) |
4.00 | ml | ||
|
|
Sodium succinate
(0.5 M) |
2.00 | ml | ||
|
|
Yeast extract
(5%) |
10.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.0 with 1 M Na2CO3 or 1 M H2SO4. Mix ingredients, except NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution, and autoclave under a N2-CO2 (80:20, v/v) atmosphere. Separately autoclave acetate solution, succinate solution and yeast extract solution. Filter-sterilize NaHCO3, Na2S x 9H2O. Aseptically and anaerobically add NaHCO3, Na2S x 9H2O, seven vitamins solution, acetate solution, succinate solution and yeast extract solution to the medium. | |||||
Trace element solution SL-12 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 3.0 | g | ||
|
|
FeSO4 x 7 H2O | 1.1 | g | ||
|
|
CoCl2 x 6 H2O | 190.0 | mg | ||
|
|
MnCl2 x 4 H2O | 50.0 | mg | ||
|
|
ZnCl2 | 42.0 | mg | ||
|
|
NiCl2 x 6 H2O | 24.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 18.0 | mg | ||
|
|
H3BO3 | 300.0 | mg | ||
|
|
CuCl2 x 2 H2O | 2.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Autoclave separately. | |||||
| 2 Adjust pH to 6.8. | |||||
Seven vitamins solution #
200
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Vitamin B12 | 10 | mg |
|
|
p-Aminobenzoic acid | 10 | mg |
|
|
D-(+)-biotin | 2 | mg |
|
|
Nicotinic acid | 20 | mg |
|
|
Calcium pantothenate | 5 | mg |
|
|
Pyridoxine hydrochloride | 50 | mg |
|
|
Thiamine-HCl x 2 H2O | 10 | mg |
|
|
Distilled water | 200 | ml |