89 .
SM MEDIUM
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Selected strain: Thiobacillus thioparus DSM 505
This strain uses the standard medium without modifications.
Equipment needed:
Filter
Final pH:
6.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 1.5 | g | ||
|
|
Na2HPO4 | 4.5 | g | ||
|
|
NH4Cl | 0.3 | g | ||
|
|
MgSO4 x 7 H2O | 0.1 | g | ||
|
|
Na2S2O3
(10%) |
100.0 | ml | ||
|
|
Trace metal solution | 5.0 | ml | ||
|
|
Distilled water | 900.0 | ml | ||
| 1 Adjust pH to 7.5. | |||||
| 2 Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium. | |||||
Trace metal solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 50.00 | g | ||
|
|
ZnSO4 x 7 H2O | 22.00 | g | ||
|
|
CaCl2 | 5.54 | g | ||
|
|
MnCl2 x 4 H2O | 5.06 | g | ||
|
|
FeSO4 x 7 H2O | 4.99 | g | ||
|
|
(NH4)6Mo7O24 x 4 H2O | 1.10 | g | ||
|
|
CuSO4 x 5 H2O | 1.57 | g | ||
|
|
CoCl2 x 6 H2O | 1.61 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 6.0 with KOH. | |||||