841 .
PROPIONISPORA MEDIUM
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Selected strain: Propionispora vibrioides DSM 13305
This strain uses the standard medium without modifications.
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.20 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.40 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
FeCl2 solution | 1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Resazurin | 0.50 | mg | ||
|
|
Yeast extract
(BD-Difco) |
1.00 | g | ||
|
|
Distilled water | 900.00 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
Fructose
(10%) |
50.00 | ml | ||
|
|
NaHCO3
(*, 5%) |
50.00 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
6.00 | ml | ||
| 2 Adjust pH to 7.0 - 7.2, if necessary. | |||||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |