58 .
INORGANIC SALTS-STARCH AGAR (ISP-4)
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Selected strain: Streptomyces antibioticus DSM 40723
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Starch
(soluble) |
10 | g | ||
|
|
K2HPO4 | 1 | g | ||
|
|
MgSO4 x 7 H2O | 1 | g | ||
|
|
NaCl | 1 | g | ||
|
|
(NH4)2SO4 | 2 | g | ||
|
|
CaCO3 | 2 | g | ||
|
|
Trace salts solution | 1 | ml | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Unadjusted pH will be 7.0-7.4. | |||||
Trace salts solution #
100
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeSO4 x 7 H2O | 0.1 | g | ||
|
|
MnCl2 x 4 H2O | 0.1 | g | ||
|
|
ZnSO4 x 7 H2O | 0.1 | g | ||
|
|
Distilled water | 100.0 | ml | ||
| 1 Comment: The premixed powder is available from Becton Dickinson & Co. as ISP Medium 4. | |||||