542 .
MODIFIED BROCK'S BASAL SALTS-YEAST EXTRACT MEDIUM
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Selected strain: Thermobacillus composti DSM 18247
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g | ||
|
|
KH2PO4 | 0.28 | g | ||
|
|
MgSO4 x 7 H2O | 0.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.07 | g | ||
|
|
FeCl3 x 6 H2O | 2.00 | mg | ||
|
|
MnCl2 x 4 H2O | 1.80 | mg | ||
|
|
Na2B4O7 x 10 H2O | 4.50 | mg | ||
|
|
ZnSO4 x 7 H2O | 0.22 | mg | ||
|
|
CuCl2 x 2 H2O | 0.05 | mg | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | mg | ||
|
|
VOSO4 x n H2O | 0.03 | mg | ||
|
|
CoSO4 x 7 H2O | 0.01 | mg | ||
|
|
Yeast extract
(BD-Difco) |
5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 9.0. | |||||
| 2 Autoclave and remove precipitate, if necessary. | |||||