176 .
ACIDIANUS BRIERLEYI MEDIUM
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Selected strain: Acidianus sp. DSM 29099
This strain uses the standard medium without modifications.
Equipment needed:
Autoclave
Final pH:
2.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.00 | g | ||
|
|
KH2PO4 | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
Ca(NO3)2 | 0.01 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.20 | g | ||
|
|
Sulfur
(powder) |
10.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 2.0 with 10 N H2SO4. Autoclave yeast extract separately. Steam sulfur for 3 hr on each of 3 successive days. | |||||