172 .
SULFOLOBUS MEDIUM (B)
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Selected strain: Saccharolobus shibatae DSM 5389
This strain uses the standard medium without modifications.
Equipment needed:
Final pH:
3.5
Select a strain (optional):
Main sol. J165 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Modified Brock's salt base solution | 1000 | ml | ||
|
|
Yeast extract
(BD-Difco) |
1 | g | ||
| 1 Adjust pH to 2.0 with 10 N H2SO4. | |||||
Modified Brock's salt base solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g |
|
|
KH2PO4 | 0.28 | g |
|
|
MgSO4 x 7 H2O | 0.25 | g |
|
|
CaCl2 x 2 H2O | 0.07 | g |
|
|
FeCl3 x 6 H2O | 2.00 | mg |
|
|
MnCl2 x 4 H2O | 1.80 | mg |
|
|
Na2B4O7 x 10 H2O | 4.50 | mg |
|
|
ZnSO4 x 7 H2O | 0.22 | mg |
|
|
CuCl2 x 2 H2O | 0.05 | mg |
|
|
Na2MoO4 x 2 H2O | 0.03 | mg |
|
|
VOSO4 x n H2O | 0.03 | mg |
|
|
CoSO4 x 7 H2O | 0.01 | mg |
|
|
Distilled water | 1000.00 | ml |