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DSMZ

DSMZ 999 . HALORHODOSPIRA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Halorhodospira neutriphila DSM 15116

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 999 #

ml
Equipment needed: Tungsten lamp
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1.00 g 1 7.348
CaCl2 x 2 H2O 0.05 g 0.05 0.34
NH4Cl 0.50 g 0.5 9.348
NaCl 100.00 g 100 1711.16
MgCl2 x 6 H2O 2.00 g 2 9.838
MgSO4 x 7 H2O 1.00 g 1 4.057
Distilled water 1000.00 ml - -
1 After autoclaving 20 min at 121°C, the following sterile solutions are added:
NaHCO3
(20%) 		10.00 ml 2 23.808
Na2S x 9 H2O
(10%) 		7.50 ml 0.75 3.123
Vitamins V7 solution (Pfennig et Trüper, 1992) 1.00 ml - -
Trace element solution SL12 1.00 ml - -
2 The pH is adjusted to pH 7.2-7.4 For better growth of pure cultures, the growth medium should be supplemented with acetate (2 mM final concentration), succinate (1 mM final concentration) and yeast extract (0.5 g/l). Pure cultures should be incubated at 30°C and light intensity of 500 lux from a tungsten lamp (16 h light, 8 h dark). Pure cultures in liquid medium can be stored in 60 ml screw cap bottles at 4°C in the dark.

Trace element solution SL12 #

1000
Solution occurs in 1 other media (first defined in Medium 998).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
H3BO3 300.0 mg 0.3 4.851
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl2 x 2 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
1 Sterilized by filtration.

Vitamins V7 solution (Pfennig et Trüper, 1992) #

1000
Solution occurs in 1 other media (first defined in Medium ).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000 ml - -
Biotine 2 mg 0.002 0.008
p-Aminobenzoate 10 mg 0.01 0.073
Thiamine 10 mg 0.01 0.038
Pantothenate 5 mg 0.005 0.023
Pyridoxamine 50 mg 0.05 0.297
Vitamine B12 20 mg 0.02 0.015
Nicotinate 20 mg 0.02 0.164
1 Sterilized by filtration.

Metadata

Last modified: 25.03.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.4
Equipment needed: Filter, Tungsten lamp
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Associated strains

All strains for this medium: 2
Halorhodospira neutriphila DSM 15116

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 100 1711.16
NaHCO3 2 23.808
MgCl2 x 6 H2O 2 9.838
KH2PO4 1 7.348
MgSO4 x 7 H2O 1 4.057
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 1 strain
temperature mesophilic 2 strains