958 .
VY/4-SWS AGAR
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Selected strain: Plesiocystis pacifica DSM 14875
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
aerobic
30 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 20.0 | g | ||
|
|
Yeast cell paste
(bakers yeast, washed in deion. water, wet weight) |
2.5 | g | ||
|
|
Agar
(Bacto ) |
15.0 | g | ||
|
|
Cyanocobalamine
(vitamin B12) |
0.5 | mg | ||
|
|
Sea water salts solution | 1000.0 | ml | ||
| 1 Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add filter sterilized cyanocobalamine solution after autoclaving and cooling. | |||||
Sea water salts solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Ferric citrate | 0.01 | g |
|
|
MgSO4 x 7 H2O | 8.00 | g |
|
|
CaCl2 x 2 H2O | 1.00 | g |
|
|
KCl | 0.50 | g |
|
|
NaHCO3 | 0.16 | g |
|
|
H3BO3 | 0.02 | g |
|
|
KBr | 0.08 | g |
|
|
SrCl2 x 6 H2O | 0.03 | g |
|
|
Sodium beta-glycerophosphate | 0.01 | g |
|
|
Trace element solution SL-4 | 1.00 | ml |
|
|
Distilled water | 1000.00 | ml |
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
EDTA | 0.5 | g |
|
|
FeSO4 x 7 H2O | 0.2 | g |
|
|
Trace element solution SL-6 | 100.0 | ml |
|
|
Distilled water | 900.0 | ml |
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
|
MnCl2 x 4 H2O | 0.03 | g |
|
|
H3BO3 | 0.30 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
CuCl2 x 2 H2O | 0.01 | g |
|
|
NiCl2 x 6 H2O | 0.02 | g |
|
|
Na2MoO4 x 2 H2O | 0.03 | g |
|
|
Distilled water | 1000.00 | ml |