897
897 .
IGNICOCCUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Ignicoccus islandicus DSM 13165
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
90 °C
Main sol. 897 #
ml
Equipment needed: Hungate tubes
Gassing station
Water bath
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 13.65 | g | 13.542 | 231.719 | |
| MgSO4 x 7 H2O | 3.50 | g | 3.472 | 14.088 | |
| MgCl2 x 6 H2O | 2.75 | g | 2.728 | 13.419 | |
| KH2PO4 | 0.50 | g | 0.496 | 3.645 | |
| (NH4)2SO4 | 0.25 | g | 0.248 | 1.877 | |
| CaCl2 x 2 H2O | 0.38 | g | 0.377 | 2.564 | |
| KCl | 0.33 | g | 0.327 | 4.391 | |
| NaBr | 0.05 | g | 0.05 | 0.482 | |
| H3BO3 | 15.00 | mg | 0.015 | 0.241 | |
|
SrCl2 x 6 H2O
(0.1% w/v) |
7.00 | ml | 0.007 | 0.026 | |
|
KI
(0.01% w/v) |
0.50 | ml | 5.0e-5 | 1.9e-4 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
|
Sulfur
(powdered) |
5.00 | g | 4.96 | 154.672 | |
| Meat extract | 1.00 | g | 0.992 | - | |
| Na2S x 9 H2O | 0.20 | g | 0.198 | 0.826 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients except sulfur, meat extract and sulfide, adjust pH to 5.0 - 5.5 with 2 N H2SO4 and sparge medium with 80% H2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials already containing the appropriate amount of sulfur, only to 30% of their volume to allow for a large head space. For sterilization heat cultivation vessels in boiling water for 1 -2 hours on each of 3 successive days. Add meat extract and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. | |||||
| 2 After inoculation pressurize the vessels with sterile 80% H2 and 20% CO2 gas mixture to 2 bar overpressure. | |||||
Metadata
| Last modified: | 27.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Archaea |
| Medium type: | Complex medium |
| Final pH: | 5.0 - 5.5 |
| Equipment needed: | Gassing station, Hungate tubes, Water bath |
| Edit in Medium builder | |
Gas composition
- 80 % H2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 3 |
| Strains with modifications: | 1 |
Ignicoccus islandicus DSM 13165
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 13.542 | 231.719 |
| Sulfur | 4.96 | 154.672 |
| MgSO4 x 7 H2O | 3.472 | 14.088 |
| MgCl2 x 6 H2O | 2.728 | 13.419 |
| Meat extract | 0.992 | - |
| Show more | ||
