88 .
SULFOLOBUS MEDIUM
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* Medium for Sulfuracidifex tepidarius DSM 104736
Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium.
Growth conditions: 3-7 days
65 °C
Equipment needed:
Autoclave
Final pH:
2.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 1.30 | g | ||
|
|
KH2PO4 | 0.28 | g | ||
|
|
MgSO4 x 7 H2O | 0.25 | g | ||
|
|
CaCl2 x 2 H2O | 0.07 | g | ||
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FeCl3 x 6 H2O | 0.02 | g | ||
|
|
Allen's trace element solution | 10.00 | ml | ||
|
|
Yeast extract
(OXOID) |
0.20 | g | ||
|
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Sulfur powder | 5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. | |||||
Allen's trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MnCl2 x 4 H2O | 180 | mg | ||
|
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Na2B4O7 x 10 H2O | 450 | mg | ||
|
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ZnSO4 x 7 H2O | 22 | mg | ||
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CuCl2 x 2 H2O | 5 | mg | ||
|
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Na2MoO4 x 2 H2O | 3 | mg | ||
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VOSO4 x 2 H2O | 3 | mg | ||
|
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CoSO4 x 7 H2O | 1 | mg | ||
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Distilled water | 1000 | ml | ||
| 1 Adjust pH of final solution to 2 with 1 N HCl. | |||||