693 .
COLUMBIA BLOOD MEDIUM
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Selected strain: Bacteroides uniformis DSM 108146
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
anaerobic
37 °C
pH 7.00
Equipment needed:
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Columbia agar base | 1000 | ml | ||
|
|
Defibrinated sheep blood | 5 | % | ||
| 1 Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). | |||||
| 2 ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freeze-dried pellets, not for cultivation. | |||||
545. TRYPTONE SOYA BROTH (TSB) #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone | 17.0 | g | ||
|
|
Soy peptone | 3.0 | g | ||
|
|
D(+)-Glucose | 2.5 | g | ||
|
|
NaCl | 5.0 | g | ||
|
|
K2HPO4 | 2.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.3. | |||||
| 2 The same as Caso Bouillon (Merck or Oxoid, Germany). | |||||
215. BHI MEDIUM #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Brain heart infusion
(Difco) |
37 | g |
|
|
Distilled water | 1000 | ml |
1. NUTRIENT BROTH #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.0. | |||||