630 .
MODIFIED THERMUS 162 MEDIUM
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* Medium for Rhodothermus marinus DSM 4253
Plus 1% NaCl
Growth conditions: 65 °C
Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 2.5 | g | ||
|
|
Tryptone | 2.5 | g | ||
|
|
Agar | 28.0 | g | ||
|
|
Nitrilotriacetic acid | 100.0 | mg | ||
|
|
CaSO4 x 2 H2O | 40.0 | mg | ||
|
|
MgCl2 x 6 H2O | 200.0 | mg | ||
|
|
Fe(III) citrate
(0.01 M) |
0.5 | ml | ||
|
|
Trace element solution | 0.5 | ml | ||
|
|
Phosphate buffer | 100.0 | ml | ||
|
|
NaCl | 10.0 | g/l | ||
|
|
Distilled water | 900.0 | ml | ||
| 1 Adjust pH to 7.2 with NaOH. Autoclave at 121°C for 15 min. Autoclave the phosphate buffer separately and then add to the medium. | |||||
Phosphate buffer #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 5.44 | g | ||
|
|
Na2HPO4 x 12 H2O | 43.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2 | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.8 | g | ||
|
|
FeCl2 x 4 H2O | 1.0 | g | ||
|
|
MnCl2 x 4 H2O | 0.5 | g | ||
|
|
CoCl2 x 4 H2O | 0.3 | g | ||
|
|
CuCl2 x 2 H2O | 50.0 | mg | ||
|
|
Na2MoO4 x 2 H2O | 50.0 | mg | ||
|
|
H3BO3 | 20.0 | mg | ||
|
|
NiCl2 x 6 H2O | 20.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 8 - 9 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4. | |||||