61 .
THERMOANAEROBACTER MEDIUM
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Selected strain: Thermoanaerobacter sp. DSM 568
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
65 °C
Equipment needed:
Autoclave
Filter
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.8 - 7.5
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Tryptone
(BD BBL) |
10.00 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
Sucrose | 10.00 | g | ||
|
|
Na2SO3 | 0.20 | g | ||
|
|
Na2S2O3 x 5 H2O | 0.08 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients (except ferrous sulfate, sucrose, sulfite, and thiosulfate), adjust pH to 6.8 - 7.5, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite, and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should be freshly prepared. | |||||