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DSMZ

DSMZ 612 . DESULFOSARCINA CETONICA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Desulfosarcina cetonica DSM 7267

This strain uses the standard medium without modifications.

Growth conditions: 3-7 days anaerobic 30 °C

Main sol. 612 #

ml
Equipment needed: Gassing station Hungate tubes Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 962 ml - -
Solution B 20 ml - -
Solution C 10 ml - -
Solution D 10 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and D are autoclaved under 100% N2 gas atmosphere. To complete the medium appropriate amounts of solutions B to D are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.2 – 7.4.

Solution A #

962
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.70 g 0.728 5.347
NH4Cl 0.30 g 0.312 5.83
Na2SO4 2.80 g 2.911 20.491
MgCl2 x 6 H2O 1.70 g 1.767 8.692
CaCl2 x 2 H2O 0.05 g 0.052 0.354
NaCl 10.00 g 10.395 177.875
Trace element solution SL-10 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.2e-4 0.002
Distilled water 960.00 ml - -

Solution B #

20
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1 g 50 471.752
Distilled water 20 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-butyrate 1.2 g 120 1090.05
Distilled water 10.0 ml - -

Solution D #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.3 g 30 124.905
Distilled water 10.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Metadata

Last modified: 19.08.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.2 - 7.4
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Desulfosarcina cetonica DSM 7267

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 9.98 170.774
Na2SO4 2.794 19.673
MgCl2 x 6 H2O 1.697 8.345
Na-butyrate 1.198 10.879
Na2CO3 0.998 9.416
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain