58 .
BIFIDOBACTERIUM MEDIUM
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* Medium for Pectinatus brassicae DSM 24661
Plus haemine (50µg/ml) plus Vit K1 plus Na-lactate (0.5%); anaerobic
Growth conditions: anaerobic
30 °C
Equipment needed:
Autoclave
Final pH:
6.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casein peptone
(tryptic digest) |
10.00 | g | ||
|
|
Yeast extract | 5.00 | g | ||
|
|
Meat extract | 5.00 | g | ||
|
|
Bacto Soytone | 5.00 | g | ||
|
|
Glucose | 10.00 | g | ||
|
|
K2HPO4 | 2.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.20 | g | ||
|
|
MnSO4 x H2O | 0.05 | g | ||
|
|
Tween 80 | 1.00 | ml | ||
|
|
NaCl | 5.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
|
Salt solution | 40.00 | ml | ||
|
|
Resazurin
(25 mg/100ml) |
4.00 | ml | ||
|
|
Haemin | 50.00 | mg/l | ||
|
|
Na-lactate | 5.00 | g/l | ||
|
|
Distilled water | 950.00 | ml | ||
| 1 The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.25 | g |
|
|
MgSO4 x 7 H2O | 0.50 | g |
|
|
K2HPO4 | 1.00 | g |
|
|
KH2PO4 | 1.00 | g |
|
|
NaHCO3 | 10.00 | g |
|
|
NaCl | 2.00 | g |
|
|
Distilled water | 1000.00 | ml |