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DSMZ

DSMZ 528 . NAPHTHALENE SULFONIC ACID MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Pseudomonas putida DSM 6884

This strain uses the standard medium without modifications.

Growth conditions: 30 °C

Main sol. 528 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.50 g 3.5 24.655
KH2PO4 1.00 g 1 7.348
NH4Cl 0.31 g 0.31 5.795
MgCl2 x 6 H2O 0.10 g 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 g 0.05 0.212
Distilled water 1000.00 ml - -
1 pH 7.0.
2 After autoclaving add 1 ml sterile trace element solution SL-4 (medium 14), 100 ml solution A and 3 ml solution B per liter.
Trace element solution SL-4 1.00 ml - -
Solution A 100.00 ml - -
Solution B 3.00 ml - -

Solution A #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Glucose 3 g 3 16.652
Sodium succinate 3 g 3 18.513
Glycerol 3 g 3 32.577
Distilled water 1000 ml - -
1 Sterilize by filtration.

Solution B #

1000
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Naphthalene sulfonic acid 2.3 g 2.3 11.046
Distilled water 1000.0 ml - -
1 Sterilize by filtration.

Trace element solution SL-4 #

1000
Solution occurs in 23 other media (first defined in Medium 14).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 0.50 g 0.5 1.487
FeSO4 x 7 H2O 0.20 g 0.2 0.719
ZnSO4 x 7 H2O 0.10 g 0.1 0.348
MnCl2 x 4 H2O 0.03 g 0.03 0.152
H3BO3 0.30 g 0.3 4.851
CoCl2 x 6 H2O 0.20 g 0.2 1.54
CuCl2 x 2 H2O 0.01 g 0.01 0.059
NiCl2 x 6 H2O 0.02 g 0.02 0.154
Na2MoO4 x 2 H2O 0.03 g 0.03 0.124
Distilled water 1000.00 ml - -
1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.

Metadata

Last modified: 19.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
Equipment needed: Filter
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Associated strains

All strains for this medium: 2
Pseudomonas putida DSM 6884

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 24.655
KH2PO4 1 7.348
NH4Cl 0.31 5.795
Glycerol 0.3 3.258
Sodium succinate 0.3 1.851
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
temperature mesophilic 2 strains