528 .
NAPHTHALENE SULFONIC ACID MEDIUM
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Selected strain: Pseudomonas putida DSM 6883
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 3.50 | g | ||
|
|
KH2PO4 | 1.00 | g | ||
|
|
NH4Cl | 0.31 | g | ||
|
|
MgCl2 x 6 H2O | 0.10 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.05 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 7.0. | |||||
| 2 After autoclaving add 1 ml sterile trace element solution SL-4 (medium 14), 100 ml solution A and 3 ml solution B per liter. | |||||
|
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Trace element solution SL-4 | 1.00 | ml | ||
|
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Solution A | 100.00 | ml | ||
|
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Solution B | 3.00 | ml | ||
Solution A #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 3 | g | ||
|
|
Sodium succinate | 3 | g | ||
|
|
Glycerol | 3 | g | ||
|
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Distilled water | 1000 | ml | ||
| 1 Sterilize by filtration. | |||||
Solution B #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Naphthalene sulfonic acid | 2.3 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Sterilize by filtration. | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
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ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||