MediaDive DSMZ Digital Diversity
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder SPARQL endpoint Unit converter Compare media Compare solutions Prediction SOON
DSMZ

DSMZ 470 . PSEUDOMONAS HALOPHILA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Medium for Halovibrio variabilis DSM 3050

This strain uses the standard medium without modifications.

Growth conditions: 28 °C

Main sol. 470 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
1 Vitamin solution is filter-sterilized and added after autoclaving. Solution A and B are autoclaved separately and combined after cooling.
2 Adjust pH to 7.0 with 6 M NaOH or HCl.

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 46.8 g 51.942 888.814
MgSO4 x 7 H2O 39.4 g 43.729 177.418
NH4Cl 1.0 g 1.11 20.749
Glycerol 5.0 g 5.549 60.261
Trace element solution SL-10 1.0 ml - -
Vitamin solution 10.0 ml - -
Distilled water 890.0 ml - -

Solution B #

100
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 1 g 10 73.484
Distilled water 100 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Vitamin solution #

1000
Solution occurs in 3 other media (first defined in Medium 11a).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.00 mg 0.002 0.008
Folic acid 2.00 mg 0.002 0.005
Pyridoxine hydrochloride 10.00 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.00 mg 0.005 0.013
Riboflavin 5.00 mg 0.005 0.013
Nicotinic acid 5.00 mg 0.005 0.041
Calcium pantothenate 5.00 mg 0.005 0.01
p-Aminobenzoic acid 1.00 mg 1.0e-3 0.007
Vitamin B12 0.01 mg 1.0e-5 7.4e-6
Distilled water 1000.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.0
Equipment needed: Autoclave, Filter
Edit in Medium builder

Associated strains

All strains for this medium: 1
Halovibrio variabilis DSM 3050

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 46.753 800.021
MgSO4 x 7 H2O 39.361 159.694
Glycerol 4.995 54.241
NH4Cl 0.999 18.676
KH2PO4 0.999 7.341
Show more
CSV
JSON

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain