463 .
NITRILOTRIACETATE MEDIUM
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Selected strain: Aminobacter aminovorans DSM 6449
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Filter
Final pH:
6.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2HPO4 x 2 H2O | 0.41 | g | ||
|
|
KH2PO4 | 0.26 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
CaCl2 x 2 H2O | 0.20 | g | ||
|
|
Trace element solution | 1.00 | ml | ||
|
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Vitamin solution | 1.00 | ml | ||
|
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Nitrilotriacetate | 1.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 pH 6.5 | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
ZnCl2 | 68.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
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H3BO3 | 62.0 | mg |
|
|
CoCl2 x 6 H2O | 120.0 | mg |
|
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CuCl2 x 2 H2O | 17.0 | mg |
|
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NiCl2 x 6 H2O | 24.0 | mg |
|
|
Na2MoO4 x 2 H2O | 24.0 | mg |
|
|
HCl , 0.05 molar | 1000.0 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Vitamin B12 | 50 | mg | ||
|
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Pantothenic acid | 50 | mg | ||
|
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Riboflavin | 50 | mg | ||
|
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Pyridoxamine hydrochloride | 10 | mg | ||
|
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Biotin | 20 | mg | ||
|
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Folic acid | 20 | mg | ||
|
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Nicotinic acid | 25 | mg | ||
|
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Nicotine amide | 25 | mg | ||
|
|
alpha-lipoic acid | 50 | mg | ||
|
|
p-Aminobenzoic acid | 50 | mg | ||
|
|
Thiamine-HCl x 2 H2O | 50 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Stir for some hours, filter sterilize the solution. | |||||