463
463 .
NITRILOTRIACETATE MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Aminobacter aminovorans DSM 6464
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Main sol. 463 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2HPO4 x 2 H2O | 0.41 | g | 0.41 | 2.888 | |
| KH2PO4 | 0.26 | g | 0.26 | 1.911 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.20 | g | 0.2 | 1.36 | |
| Trace element solution | 1.00 | ml | - | - | |
| Vitamin solution | 1.00 | ml | - | - | |
| Nitrilotriacetate | 1.00 | g | 1 | 5.232 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 6.5 | |||||
Solution occurs in 1
other media (first defined in Medium 463).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 |
| ZnCl2 | 68.0 | mg | 0.068 | 0.499 |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 |
| H3BO3 | 62.0 | mg | 0.062 | 1.003 |
| CoCl2 x 6 H2O | 120.0 | mg | 0.12 | 0.924 |
| CuCl2 x 2 H2O | 17.0 | mg | 0.017 | 0.1 |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 |
| Na2MoO4 x 2 H2O | 24.0 | mg | 0.024 | 0.099 |
| HCl , 0.05 molar | 1000.0 | ml | - | - |
Solution occurs in 15
other media (first defined in Medium 461).
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Vitamin B12 | 50 | mg | 0.05 | 0.037 | |
| Pantothenic acid | 50 | mg | 0.05 | 0.228 | |
| Riboflavin | 50 | mg | 0.05 | 0.133 | |
| Pyridoxamine hydrochloride | 10 | mg | 0.01 | 0.049 | |
| Biotin | 20 | mg | 0.02 | 0.082 | |
| Folic acid | 20 | mg | 0.02 | 0.045 | |
| Nicotinic acid | 25 | mg | 0.025 | 0.203 | |
| Nicotine amide | 25 | mg | 0.025 | 0.205 | |
| alpha-lipoic acid | 50 | mg | 0.05 | 0.242 | |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 | |
| Thiamine-HCl x 2 H2O | 50 | mg | 0.05 | 0.134 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Stir for some hours, filter sterilize the solution. | |||||
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.5 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 12 |
Aminobacter aminovorans DSM 6464
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Nitrilotriacetate | 1 | 5.232 |
| MgSO4 x 7 H2O | 1 | 4.057 |
| Na2HPO4 x 2 H2O | 0.41 | 2.888 |
| KH2PO4 | 0.26 | 1.911 |
| CaCl2 x 2 H2O | 0.2 | 1.36 |
| Show more | ||
Cultivation metadata from 12 strains
| pH | 4 - 6 | 1 strain |
| pH | 6 - 8 | 1 strain |
| pH | 8 - 10 | 1 strain |
| phyla | 1 | 12 strains |
| temperature | mesophilic | 12 strains |